Figure 7.

GPR108 Is Required for Transduction in Primary Cells and In Vivo

(A) Mouse embryonic fibroblast (MEF) cells derived from WT or GPR108 KO mice transduced with the indicated serotype. The same transduction protocol was used as described in Figure 2B. (B) In vivo bioluminescence of WT or GPR108 KO mice injected retro-orbitally with 10¹¹ VG/mouse of CMV.Luciferase.SVPA packaged AAV8, rh32.33, or AAV5 at 14 days post-injection. (C) Quantification of bioluminescence at 7, 14, 28, or 56 days post-injection from mice in (B). n = 3 mice per group; uninjected is maximum observed luminescence from uninjected mice across all time points. Error bars represent SEM.