Figure 3.

Quantifiers of Transcriptome Remodeling and Gene Therapy Efficacy in XLMTM Dogs

(A) Venn diagram representing the intersections of differentially expressed gene populations obtained after comparison of untreated XLMTM, rAAV-treated XLMTM, and healthy dogs. Focus is made on the Rescued and the Resistant two-circle domains in specific insets and on the central domain containing Partially Rescued and Worsened genes. Exemplar gene-expression levels are plotted on a line for the three groups: healthy, XLMTM, and rAAV-treated (AAV) dogs. n.s., non-significant gene expression difference; *, significant difference. (B and C) Evolution of the described metrics in the Sub-therapeutic and the Therapeutic groups of dogs—as defined in the text—in the Biceps (B) and the Vastus (C) muscles. The initial DE gene set in XLMTM dogs is represented as a blue ring, and its counterpart in healthy controls as a green ring. In rAAV-treated dogs, the areas corresponding to the above-described metrics have an arc length directly proportional to the proportion of initial DE genes presenting the corresponding expression profile.