Table 1.
Details of the Primers, Probe, and Gene-Expression Arrays Used in the qRT-PCR Validation Experiment
| RPL32 | Forward primer |
5′-TGGTTACGGGAGCAACAAGAAA-3′ |
|---|---|---|
| Reverse primer |
5′-GCACATCAGCAGCACTTCA-3′ |
|
| Probe | 5′-TGCTGCCCAGTGGCTTCTGG-3′ - VIC-QSY | |
| CHRNA1 | Thermo Fisher Scientific – TaqMan Gene Expression - Cf02621957_m1 - FAM/MGB-NFQ | |
| CHRND | Thermo Fisher Scientific – TaqMan Gene Expression - Cf02691404_m1 - FAM/MGB-NFQ | |
| CHRNG | Thermo Fisher Scientific – TaqMan Gene Expression - Cf02704147_m1 - FAM/MGB-NFQ | |
| IGF2 | Thermo Fisher Scientific – TaqMan Gene Expression - Cf02693069_m1 - FAM/MGB-NFQ | |
| FST | Thermo Fisher Scientific – TaqMan Gene Expression - Cf02645950_m1 - FAM/MGB-NFQ | |
| MSTN | Thermo Fisher Scientific – TaqMan Gene Expression - Cf02704228_m1 - FAM/MGB-NFQ | |
| ANGPTL2 | QIAGEN - qRT-PCR2 primer assay for dog ANGPTL2 – product 330001 | |