Figure 7.

Regenerative Effect of Klotho-Engineered Murine uEVs

(A) Creatinine and BUN levels and number of tubular hyaline casts and tubular necrosis in AKI mice treated with vehicle, wild-type (muEV, 2.0 × 10⁸/mouse), Klotho null murine uEVs (muEV KL^−/−, 2.0 × 10⁸/mouse) (n = 6), and Klotho null murine uEVs engineered with human recombinant Klotho (muEV KL^−/− + KLOTHO, 2.2 × 10⁶/mouse) at day 3 after damage (n = 3). Data are expressed as mean ± SEM; ANOVA with Dunnett’s multicomparison test was performed: *p < 0.05 or **p < 0.001 versus vehicle. (B) Representative micrographs of H&E of renal tissue from AKI mice treated with wild-type (muEV), Klotho null murine uEVs (muEV KL^−/−), and Klotho null murine uEVs engineered with human recombinant Klotho (muEV KL^−/− + KLOTHO) at day 3 after damage. Original magnification ×200.