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. 2019 Oct 24;28(2):572–586. doi: 10.1016/j.ymthe.2019.10.015

Figure 3.

Figure 3

miR24-2 Promotes the Expression of Nanog in Human Liver Stem Cells

(A) The RIP using anti-KLF7, anti-C-myc, anti-Epcam. The HULC sequence was designed to amplify the HULC by RT-PCR. IgG RNA immunoprecipitation (RIP) was used as a negative control. (B) Immunoprecipitation using anti-C-myc, anti-Epcam, and anti-KLF4, respectively. (C) The coIP with anti-C-myc, anti-Epcam, and anti-KLF4, respectively. (D) ChIP using anti-C-myc, anti-Epcam, and anti-KLF4. IgG ChIP was used as a negative control. (E) The ChIP using anti-C-myc, anti-Epcam, and anti-KLF4. (F) The pEZX-MT-Nanog-Luc luciferase reporter gene activity was assayed. (G) The pEZX-MT-Nanog-Luc luciferase reporter gene activity was detected. **p < 0.01, *p < 0.05. (H) The Nanog was analyzed by RT-PCR and western blot. β-actin was used as an internal reference gene. (I) The Nanog was analyzed by RT-PCR and western blotting. β-actin was used as an internal reference gene.