Figure 7.

miR24-2 Promotes the Expression of the Tyrosine Protein Kinase Sarcoma Gene Src by Enhancing PKM1 in hLCSCs

(A) The coIP with anti-PKM1 was performed and the precipitates were analyzed by western blotting with anti-LC3 or anti-P62. IgG coIP was used as a negative control. (B) The coIP with anti-PKM1. (C) Western blotting using anti-PKM1. (D) Western blotting with anti-PKM1was performed. β-actin as an internal reference gene. (E) The total protein was subjected to western blotting using anti-PKM1. (F) Western blotting was performed using anti-PKM1. β-actin was used as an internal reference gene. (G) ChIP was performed using anti-PKM1. IgG ChIP was used as a negative control. (H) The binding ability of PKM1 to the Src promoter-enhancer loop was analyzed by chromosomal conformation capture (3C)-ChIP. IgG ChIP-3C was used as a negative control and the products amplified by independent primers designed by Src promoter and enhancer were used as internal reference (INPUT). (I) ChIP was performed. (J) 3C-ChIP was performed. (K) The pGL4-Src-Luc luciferase reporter activity was detected. (L) The pGL4-Src-Luc luciferase reporter gene activity was detected. (M) RT-PCR was used to detect Src. β-actin was used as an internal reference gene. (N) RT-PCR was used to detect Src. β-actin was used as an internal reference gene. (O) Western blotting was performed using anti-Src. (P) Western blotting was performed using anti-Src. (Q) RT-PCR was used to detect Src. (R) RT-PCR was used to detect Src. β-actin was used as an internal reference gene. (S) Western blotting was performed using anti-Src. (T) Western blotting was performed using anti-Src. β-actin was used as an internal reference gene.