Figure 8.

Src Affects miR24-2-Induced Malignant Growth of hLCSCs

(A) The miR24-2 was detected by northern blot. U6 was used as an internal reference gene. (B) The miR24-2 was detected by RT-PCR. (C) Western blotting was used to detect the Src. β-actin as an internal reference gene. (D) The cell proliferation ability was determined by the CCK8 method. (E) Determination of the S phase percentage of hLCSCs cells by BrdU staining. (F) Determination of plate colony forming ability of cells. (a) Photograph of plate colonies. (b) The analysis of cell plate colony formation rate. (G) The assay of cell sphere formation ability. (H) The hLCSCs were inoculated into the BALB/c nude mice for 1 month. Photography of xenografts. (I) Comparison of the size (g) of transplanted tumors in nude mice (n = 6). **p < 0.01,*p < 0.05. (J) The comparison of appearance time (days) of transplanted tumors in nude mice (n = 6). **p < 0.01, *p < 0.05. (K) RT-PCR was used to detect HULC, and western blotting was used to detect the expression of Src, PRMT7, C-myc, Nanog, Beclin1, H4K16Ac, and PKM1. β-actin was used as an internal reference gene. (L) The cell proliferation ability was determined by the CCK8 method. (M) The analysis of cell plate colony formation rate. (N) The assay of cell sphere formation ability. **p < 0.01, *p < 0.05.