Table 1.
Base case process assumption for limonene biomanufacturing process.
| Parameters | Value | Source |
|---|---|---|
| Limonene productivity (kg/(m3·h)) | 0.1–1 | assumed |
| Limonene recovery (fermenter) (% w/w) | optimisable | – |
| Limonene recovery (scrubber) (% w/w) | optimisable | – |
| Operating Mode | Fed-batch | assumed |
| Target limonene purity (wt %) | 96 | assumed |
| Process energy utilisation efficiency (%) | 60 | assumed |
| Initial reactor working volume (m3) | 100 | assumed |
| Batch time (h) | 50 | assumed |
| Down time (h) | 5 | assumed |
| Initial glucose concentration (kg/m3) | 100 | assumed |
| Glucose concentration in the feed (kg/m3) | 800 | assumed |
| Glucose distribution to limonene (w/w) | 0.45 | assumed |
| Glucose distribution to acetic acid (w/w) | 0.05 | assumed |
| Biomass synthesis/respiration carbon split ratio | 0.47 | (Wu and Maravelias, 2018) |
| Specific power input (i.e. P/V ratio) (W/L) | 1.5 | (Benz, 2008) |
| Limonene recovery (distillation) | 99 | assumed |
| Operating temperature and pressure in the fermentation and product recovery unit operation | 30 °C, 1 atm | assumed |