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. 2020 Feb 4;31(2):391–405.e8. doi: 10.1016/j.cmet.2019.10.015

Figure 4.

Figure 4

TEPP-46 Limits Th17 Cell Polarization

Murine CD4+CD62+ T cells were activated in vitro for 3 days with CD3/CD28 antibodies under Th17-polarizing conditions in the presence of DMSO (CTRL condition), TEPP-46 50 μM, or 100 μM.

(A) Flow cytometry evaluation of IL-17A and TNF-α production by Th17 cells after intracellular cytokine staining. Left, representative plots showing reduced IL-17A and TNF-α production by TEPP-46 treated cells. Right, quantification of the percentage of IL-17A/TNF-α-producing cells and of IL-17A/TNF-α MFI in CTRL versus TEPP-46-treated cells (n = 6–9 from 3 independent experiments).

(B and C) Quantification of the mRNA levels of Th17 signature cytokines (B) and transcription factors (C) by qRT-PCR (n = 6–8 from 3–4 independent experiments). For all panels, data are the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, or ∗∗∗∗p < 0.0001 compared to CTRL condition, by one-way ANOVA with Dunnett's post-hoc test.