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. 2020 Feb 4;31(2):391–405.e8. doi: 10.1016/j.cmet.2019.10.015

Figure 5.

Figure 5

TEPP-46 Constrains Th1 Cell Development

Murine CD4+CD62+ T cells were activated in vitro for 3 days with CD3/CD28 antibodies under Th1-polarizing conditions in the presence of DMSO (CTRL condition) or TEPP-46 50 μM or 100 μM.

(A) Flow cytometry evaluation of IFN-γ and TNF-α production by Th1 cells after intracellular cytokine staining. Left, representative plots showing reduced IFN-γ and TNF-α production by TEPP-46 treated cells. Right, quantification of the percentage of IFN-γ/TNF-α-producing cells and of IFN-γ/TNF-α MFI in CTRL versus TEPP-46-treated cells (n = 5–6 from 4 independent experiments).

(B and C) Quantification of Tnfa/Ifng (B) and Tbx21/Eomes (C) mRNA levels by qPCR (n = 5–6 from 3 independent experiments). For all panels, data are the mean ± SD. ∗∗∗p < 0.001 or ∗∗∗∗p < 0.0001 compared to CTRL condition, by one-way ANOVA with Dunnett's post-hoc test.