Figure 3.

FABP5 Inhibition of Fully Differentiated Tregs Increases Suppression and Expression of the Regulatory Cytokine IL-10

Treg suppression assays were performed following acute FABP5 blockade.

(A) Quantification of the mean suppression (±SEM) of responder effector cells from in vitro-differentiated Tregs following overnight BMS309403 treatment (n = 4). Results represent five independent experiments.

(B) Quantification of mean suppression (±SEM) from human Tregs following BMS309403 treatment (n = 4). Results represent the combined results from 4 independent donors.

(C) Quantification of mean suppression (±SEM) from ex vivo Tregs following 30 min BMS309403 treatment (n = 4). Results represent two independent experiments.

(D) Representative histograms and mean (±SEM) quantification of CD25 and ICOS expression on Tregs following acute FABP5 blockade (n = 4). Results represent six independent experiments.

(E) Representative histograms and mean (±SEM) quantification of CD25 and ICOS expression on Tregs following lentiviral knockdown of Fabp5. Results represent two independent experiments.

(F) Mean (±SEM) expression of Il10 gene expression measured by qPCR (n = 4).

(G) Mean (±SEM) protein expression of IL-10 in Tregs after overnight BMS309403 treatment (n = 5). Results represent two independent experiments. Gating controls are depicted in red.

(H) Mean quantification (±SEM) of suppression from in vitro-generated Tregs following overnight BMS309403 treatment in the presence or absence of anti-IL-10 antibody (αIL10) (n = 4). Results represent four independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005, ^∗∗∗∗p < 0.001. P values were calculated using a two-way ANOVA with Bonferroni correction (A, B, C, and H) or a two-tailed, unpaired t test (D, E, F, and G).