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. 2019 Dec 6;28(2):441–451. doi: 10.1016/j.ymthe.2019.11.029

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Histological and Immunohistochemical Staining Evaluated

(A) H&E staining. (B) Osteopontin (OPN) staining. (C) Bone sialoprotein (BSP) staining. (D) TRAP staining. (E) Statistical analysis of staining results (pixels count/field). The rats were sacrificed at 8 weeks postimplantation, and the calvarial bones were removed and sectioned for histological or immunohistochemical staining. (F) Statistical analysis of number of TRAP⁺ osteoclasts. Red arrowheads indicate OPN; black arrowheads indicate BSP. Open arrowheads indicate TRAP. F, fibrous tissue; B, bone tissue. For quantitative analysis, five fields from each section were analyzed using ImageJ software. Representative images of five animals are shown and the data represent the mean ± SD. Student’s t test was used to analyze statistical significance.