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. 2019 Dec 14;28(2):561–571. doi: 10.1016/j.ymthe.2019.11.030

Figure 1.

Figure 1

Transgene Integration in Master Cell Bank Clone 73

(A) TLA sequence coverage of the TEG001 insert in MCB across the pMP71:TCRγ5-T2A-δ5 plasmid reference sequence. The gray vertical bars represent the number of NGS reads that align to each plasmid position and indicate the integrated TEG001 transgene sequence. The coverage generated with primer set 1 is depicted in the top panel and with primer set 2 in the bottom panels. The positions of the primers are represented by black arrows below each coverage profile. The y axis is limited to 100×. (B) TLA sequence coverage across the human genome using the TEG001 insert specific primer set 2. The different chromosomes are indicated on the y axis, and the chromosomal position on the x axis. Encircled are the regions containing the TEG001 transgene integration sites. Locations in smaller circles show less obvious peaks, but fusion sequences to these positions do confirm the integration sites there.