Table 1.

Small Sequence Variants

		Position
		415	568	578	606	3552
		Reference Nucleotide
		C	T	T	A	C
		Mutation
		G	C	C	+1C	G
Plasmid	coverage	8,655	7,316	7,327	7,273	5,208
	% mutation	0	0	0	0	0
MCB	coverage	2,051	1,750	1,785	1,802	336
	% mutation	10	9	14	10	8
TEG001-28	coverage	3,389	2,039	2,032	1,971	571
	% mutation	11	15	19	12	10
TEG001-31	coverage	2,810	17,60	1,727	1,707	480
	% mutation	13	17	20	10	11
TEG001-32	coverage	4,857	3,064	3,013	2,939	851
	% mutation	12	16	17	9	14
TEG001-33	coverage	42,660	33,494	33,461	32,897	7,017
	% mutation	12	19	17	8	11
TEG001-32_22w	coverage	20,881	16,291	17,290	16,880	3,543
	% mutation	8	23	14	5	8
TEG001-33_24w	coverage	2,516	2,299	2,360	2,316	396
	% mutation	8	3	2	3	7

Position of the five nucleotide variants and their allele frequencies in the plasmid (as control), the MCB, the TEG001 drug product samples, and the cultured TEG001 samples are shown. Position: position in the reference sequence at which the variant is found; reference nucleotide: the nucleotide present in the reference sequence at this position; mutation: the variant nucleotide identified at the indicated position, +1C indicates the insertion of 1 C after the reference A. Coverage at this position is the average between the two primer sets. % mutation, mutant allele frequency; that is, the percentage of reads that contained the variant and the average of results of the two primer sets.