KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti phospho-CHKI | Cell Signaling Technology | 2348; RRID: AB_331212 |
| Mouse anti phospho-CHK2 | BD Biosciences | 611570; RRID: AB_399016 |
| Mouse anti γ-H2AX | Millipore | 05–636; RRID: AB_309864 |
| Mouse anti-TRF2 | Millipore | 05–521; RRID: AB_2303145 |
| Mouse anti-Myc | Millipore | 05–724, RRID: AB_309938 |
| Rabbit anti-RAD51 | Santacruz | sc-8349, RRID: AB_2253533 |
| Mouse anti-BRCA1 | Santacruz | sc-6954, RRID: AB_626761 |
| Rabbit anti-BARD1 | Santacruz | sc-11438; RRID: AB_2061240 |
| Rabbit anti-CTIP | Santacruz | sc-22838, RRID: AB_2175257 |
| Mouse anti-SMARCAL1 | Santacruz | sc-376377, RRID: AB_10987841 |
| Rabbit anti-PCNA | Santacruz | sc-7907, RRID: AB_2160375 |
| Mouse anti-GFP | Santacruz | sc-9996; RRID: AB_627695 |
| Rabbit anti-53BP1 | Santacruz | sc-22760; RRID: AB_2256326 |
| Rabbit anti-EXO1 | Santacruz | sc-33194, RRID: AB_2101433 |
| Mouse RAD52 | Santacruz | sc-365341, RRID: AB_10851346 |
| Mouse anti-Flag | Sigma-Aldrich | F3165; RRID: AB_259529 |
| Mouse anti-HA | Sigma-Aldrich | H3663; RRID: AB_262051 |
| Rabbit anti-V5 | Sigma-Aldrich | V8137, RRID: AB_261889 |
| Mouse anti-γ-Tubulin (clone GTU-488) | Sigma-Aldrich | T6557; RRID: AB_477584 |
| Mouse anti-Myc agarose beads | Sigma-Aldrich | A7470; RRID: AB_10109522 |
| Mouse anti-Flag M2 affinity Gel | Sigma-Aldrich | A2220; RRID: AB_10063035 |
| Mouse anti-HA agarose beads | Sigma-Aldrich | A2095, RRID: AB_257974 |
| Rabbit anti-DONSON | Sigma-Aldrich | AV45862, RRID: AB_1847828 |
| Rabbit anti-DONSON | Invitrogen | PA5–61865, RRID: AB_2640751 |
| Rabbit anti-Claspin | Abcam | ab3720, RRID: AB_2245123 |
| Rabbit anti-Claspin | Novus | NB100–248, RRID: AB_2082897 |
| Rabbit anti-Claspin | Bethyl Laboratories | A300–266A, RRID: AB_155895 |
| Rabbit anti phospho-RPA32 (S4/S8) | Bethyl Laboratories | A300–245A, RRID: AB_210547 |
| Mouse anti PCNA | Bio-Rad | VMA00018 |
| Rabbit anti-mTRF2 | Karlsedar Lab | N/A |
| Streptavidin-Agarose resin | Thermo Scientific | 20359 |
| Bacterial and Virus Strains | ||
| XL1-Blue Supercompetent cells | Agilent Technologies | 200518 |
| XL10-Gold Ultracompetent Cells | Agilent Technologies | 200315 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DNA-PKcs inhibitor NU7441 | Selleckchem | S2638 |
| Critical Commercial Assays | ||
| Site-directed mutagenesis | Stratagene | 200521 |
| TRAPeze Telomerase Detection Kit | Fisher Scientific | S7700 |
| Experimental Models: Cell Lines | ||
| Human NBS-ILB1 cells | Falck et al., 2012, EMBO Reports. | N/A |
| U2OS | ATCC | N/A |
| 293T | ATCC | CRL-3216, RRID: CVCL_0063 |
| Nbs1−/− MEFs | Matsuura et al., 2004, Adv Biophys. | N/A |
| Apollo/SNM1B−/− MEFs | Lam et al., 2010, EMBO J. | N/A |
| G1 mTR−/− MEFs | This paper | N/A |
| MMTV-Cre+; Pot1aF/Δ; p53Δ/Δ MEFs | Gu et al., 2017 | N/A |
| MMTV-Cre+; Pot1aΔ/Δ; p53Δ/Δ MEFs | ||
| Experimental Models: Organisms/Strains | ||
| MMTV-Cre+; Pot1aF/F; p53F/F mouse tumor model | Gu et al., 2017 | N/A |
| Oligonucleotides | ||
| Biotin-Tel-G (IIAGGG)6 | This paper | N/A |
| Biotin-Tel-C (CCCTAA)6 | This paper | N/A |
| Biotin Oligo (dT)6 | This paper | N/A |
| TelC-Cy3 (CCCTAA)3 | PNABio | F1002 |
| TelG-FAM (TTAGGG)3 | PNABio | F1005 |
| Recombinant DNA | ||
| pBabe puro Myc-hTRF2ΔBΔM | Rai et al., 2017, Molecular Cell | N/A |
| pQCXIP puro Flag-hTPP1ΔRD | Rai et al., 2017, Molecular Cell | N/A |
| pQCXIP puro HA-hTPP1ΔRD | Rai et al., 2017, Molecular Cell | N/A |
| pQCXIP puro HA-mTPP1ΔRD | Rai et al., 2017, Molecular Cell | N/A |
| pQCXIP puro Flag-mTPP1ΔRD | Rai et al., 2017, Molecular Cell | NA |
| pQCXIP puro Flag-mNBS1WT | Rai et al., 2017, Molecular Cell | N/A |
| pQCXIP Flag-TIN2A110R | Hu et al., 2017, Cell Res. | N/A |
| pQCXIP Myc-mPOT1a and OB fold mutant mPOT1aF62A | Wu et al., 2006, Cell | N/A |
| pCDNA3.1 Myc-TRF2 | Chen et al., 2011, NSMB | N/A |
| pEGFP-EXO1 | Tomimatsu et al., 2014, Nature Comm. | N/A |
| pLenti V5-EXO1 | Tomimatsu et al., 2014, Nature Comm. | N/A |
| Fucci mKO-CTD1 | Sakaue-Sawano et al., 2008, Cell | N/A |
| Fucci mAG-Geminin | Sakaue-Sawano et al., 2008, Cell | N/A |
| pGEX4T1 Human N-Terminal, C-Terminal, WT and Claspin 3A mutant | Lindsey-Boltz et al., 2009, JBC | N/A |
| pQCXIP HA-hClaspin WT and mutants | This paper | N/A |
| pQCXIP HA-mClaspin WT and mutants | This paper | N/A |
| pQCXIP HA-mPCNA | This paper | N/A |
| pCMV6 Myc-mPCNA | Origene | RC209379 |
| pQCXIP Myc hDonson | This paper | N/A |
| pEGFP-DONSON and disease mutants | Reynolds et al., 2017 | N/A |
| Flag-ATRIP | Lee Zou (Harvard Medical School) | N/A |
| pRetroSuper shTrf2 | Deng et al., 2009 | N/A |
| pMKO.1 shBRCA1 | Xiaohua Wu (Scrips Research Institute) | N/A |
| pRetroSuper shParp1 | Madalena Tarsounas (University of Oxford) | N/A |
| pRetroSuper shLigase 3 | Madalena Tarsounas | N/A |
| pRetroSuper shRad51 | Madalena Tarsounas | N/A |
| pGIPZ shClaspin | Zhenkun Lou (Mayo Clinic) | N/A |
| pGIPZ shRad52 | Ryan Jensen (Yale University) | V2LHS_171206 |
| V3LHS_376616 | ||
| PlK.01 Lenti shPolq | Agnel Sfeir (NYU) | N/A |
| PlK.01 Lenti shCtip | Sigma-Aldrich | TRCN0000305376 |
| PlK.01 Lenti shExo1 | Sigma-Aldrich | TRCN0000218614 |
| TRCN0000238466 | ||
| TRCN0000238468 | ||
| PlK.01 Lenti shClaspin | Sigma-Aldrich | TRCN0000193573 |
| TRCN0000175992 | ||
| TRCN0000193398 | ||
| PlK.01 Lenti shPcna | Sigma-Aldrich | TRCN0000294872 |
| TRCN0000294805 | ||
| TRCN0000287377 | ||
| PlK.01 Lenti shDonson | Sigma-Aldrich | TRCN0000377075 |
| TRCN0000249773 | ||
| TRCN0000201175 | ||
| PlK.01 Lenti shPCNA | Sigma-Aldrich | TRCN0000003862 |
| TRCN0000003864 | ||
| PlK.01 Lenti shDONSON | Sigma-Aldrich | TRCN0000275364 |
| TRCN0000275365 | ||
| TRCN0000275367 | ||
| Software and Algorithms | ||
| GraphPad Prism Software (Version 7) | San Diego, CA | RRID:SCR_002798 |
| NIS-Elements BR (Verson3.2) | Nikon | RRID:SCR_002776 |
| Origin 7 software | OriginLab | N/A |
| ImageQuantTL | GE Healthcare | RRID:SCR_014246 |
| FlowJo | FlowJo LLC | RRID:SCR_008520 |