Fig. 2.

Establishment of the MSG-induced excitotoxicity slice model. Percent cytotoxicity was calculated from LDH absorbance for a slices treated with gradient of MSG concentrations (n = 18; mean ± SEM), and b organotypic hippocampal (OHC) (n = 3; mean ± SEM) and c organotypic whole hemisphere (OWH) (n = 6 NaCl, LPS; n = 18 NT, MSG; mean ± SEM) ex vivo slice cultures treated with various exposures, referenced to the 24 h cumulative 1% TX LDH absorbance as 100%. Representative photos of d OHC slice cultures and e OWH slice cultures