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. 2020 Feb 5;16:12. doi: 10.1186/s13007-020-0562-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Successful replacement of cotyledons does not result in deficiency in biomass production. The impact of cot-grafting on plant development was investigated by evaluating biomass production at two different developmental stages after grafting. a Illustration of the biomass evaluation workflow. b–d Fresh weight measurements of whole seedlings 15 days after sowing (DAS; b). Above ground biomass of single plants 35 DAS (c), and number of rosette leaves 35 DAS (d). Cot-grafted plants (grafted cotyledon—GC) were compared to three controls: intact plants with two cotyledons (2C), plants without cotyledons (0C) and plants with one cotyledon removed (1C). N = 15; different letters denote statistically significant differences as assessed by 1-way ANOVA followed by a Tukey HSD test (p < .05). SUC, sucrose; ATS, A. thaliana solution medium