Fig. 5.

Putative tumor-derived circulating miRNA that modulates the TME. Kaplan–Meier curves for 5-year overall survival rate of melanoma patients from G2 and G3 according to High (≥ average expression) or Low (< average expression) expression of a mir-149 (p = 0.16, log-rank test) and bNLRC5 (p = 0.0047, log-rank test). c Violin plot showing the expression of NLRC5 by cell type based on melanoma SCRS data. Asterisks inform on Mann–Whitney p values using the malignant cell type as reference. d Boxplots of the expression levels of mir-149, NLRC5 and genes involved in the antigen presentation pathway (TAP1, B2M, HLA-A/B/C) on G2 (green) and G3 (pink). Asterisks inform on Mann–Whitney test p values. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. e Kaplan–Meier curves for 5-year overall survival rate of melanoma patients from G2 and G3 according to High (≥ average expression) or Low (< average expression) expression of a mir-1914 (p = 0.39, log-rank test) and fHLA-F (p = 0.027, log-rank test). g Violin plot showing the expression of HLA-F by cell type based on melanoma SCRS data. The malignant cell type was used as reference for MW test. h Boxplots of miR-1914 expression levels (log 10 normalized beta values) in extracellular vesicles extracted from plasma samples. MW test was used to compare pairwise means from all groups. **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001