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. 2020 Feb 4;21:6. doi: 10.1186/s12868-020-0554-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Proximity ligation assay showing pre- and postsynaptic localization of ADAM10 and BACE1 in adult rat brain. Adult rat brain hippocampal sections were labelled with primary antibodies (anti-ADAM10 or anti-BACE1 made in rabbit and anti-synaptophysin or anti-PSD-95 made in mouse) followed by secondary oligonucleotide-linked probes. Each PLA signal (brown dot) denotes two proteins within 40 nm distance from each other; a ADAM10 and synaptophysin, b ADAM10 and PSD-95, c BACE1 and synaptophysin and d BACE1 and PSD-95. In the negative control samples only one primary antibody (anti-synaptophysin (e), anti-PSD-95 (f), anti-ADAM10 (g) or anti-BACE1 (h)) was used. Brain tissue and cell nuclei were visualized by a nuclear stain solution containing Mayer´s haematoxylin. Each experiment was performed five times (technical replicates) and representative images are shown. Scale bar 20 µm