Fig. 2.

Proximity ligation assay showing pre- and postsynaptic localization of APP in adult rat brain. Adult rat brain hippocampal sections were subjected to PLA and each signal (brown dot) generated denotes two proteins within 40 nm distance from each other. a APP and synaptophysin, b APP and PSD-95. c Negative control with no primary antibodies. Brain tissue and cell nuclei were visualized by a nuclear stain solution containing Mayer´s haematoxylin. Each experiment was performed three times (technical replicates) and representative images are shown. Scale bar 20 µm