Fig. 6.

TTBK1 suppresses axonal elongation in Rho-dependent manner. a Primary cultured mouse cortical neurons (green) were cultured on the PDL-coated coverslip in 24-well tissue culture plate, transfected with DNA plasmids expressing GFP, TTBK1, and/or DN-Rho (0.5 μg) at 5 div and fixed at 8 div. Blue represents Dapi counterstain. b-c Neurite length (b) and the number of branches from primary axons (c) in GFP⁺ neurons were determined. Results were representative of 3 independent experiments. The error bars indicate SEM. * denotes p < 0.05 as determined by one-way ANOVA with Tukey post hoc (N = 10 cells per group). ANOVA: F (3, 8) = 36.273 (p < 0.0001). Scale bars represent 100 μm