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. 2020 Feb 4;17:47. doi: 10.1186/s12974-020-1726-7

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Hypoxia promotes exosome release from MSCs. a Morphology of Exos and HExos under TEM. b NTA analysis of Exos and HExos revealed that exosomes from the two groups exhibit similar size ranges (50–150 nm). c Western blot analysis of exosomal proteins including TSG101, CD9, CD63 and CD81. d Exosome protein concentration in the two groups using the BCA assay. e Uptake of the red fluorescence dye Dil-labeled Exos and HExos into BV2 microglia. f Statistical evaluation of fluorescence intensities in the two groups. ^*P < 0.05