Fig. 7.

HExos shifted polarization from M1 to M2 in BV2 and primary microglia by shuttling miR-216a-5p in vitro. a The concentrations of pro-inflammatory and anti-inflammatory cytokines in BV2 microglia in the miR-NC^OE-HExos and miR^OE-HExos groups. b The mRNA expression levels of the M1- and M2-related genes were detected by qRT-PCR in BV2 microglia in the miR-NC^OE-HExos and miR^OE-HExos groups. c The concentrations of pro-inflammatory and anti-inflammatory cytokines in primary microglia in the miR-NC^KD-HExos and miR^KD-HExos groups. d The mRNA expression levels of M1- and M2-related genes were detected by qRT-PCR in primary microglia in the miR-NC^KD-HExos and miR^KD-HExos groups. e and f The protein expression levels of M1- and M2-related genes were detected by western blot in BV2 and primary microglia in the different groups. g and h Quantification of the immunofluorescence intensity of iNOS and Arg1 from five different fields in each group. ^*P < 0.05