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. 2020 Feb 4;8:11. doi: 10.1186/s40478-020-0886-0

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Representative images of locus coeruleus in control and PSP cases. a Photographs of pons sections including the locus coeruleus (arrows). b Micrographs showing the location of locus coeruleus near the floor of the 4th ventricle in a control and PSP case (red arrows). Stitched images from micrographs taken using a 4X objective. c Micrographs taken using a 20X objective, red arrows indicate representative examples of pigmented neurons of the locus coeruleus and hollow red arrows indicate examples of pigmented neurons with tau-inclusions. b and c Sections were stained for hyperphosphorylated tau (AT8) in purple using methyl green as counterstain, endogenous neuromelanin shown in brown