Table 3.
Statistical analyses of extracellular vesicle mRNA markers in clinical samples
| Biomarker(s) | n | Cut-offa | AUC | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|
| MYC | 1 | 2.72 | 0.56 | 40 | 100 | 60 |
| VEGF | 1 | 6.76 | 0.78 | 80 | 80 | 80 |
| CDX2 | 1 | NA | NA | NA | NA | NA |
| CD133 | 1 | 1.275 | 0.86 | 80 | 100 | 87 |
| CEA | 1 | NA | NA | NA | NA | NA |
| CK19 | 1 | 4.85 | 0.54 | 40 | 100 | 60 |
| EpCAM | 1 | NA | NA | NA | NA | NA |
| CD24 | 1 | 3.06 | 0.63 | 50 | 80 | 60 |
| VEGF+CK19 | 2 | 8.305 | 0.94 | 100 | 80 | 93 |
| VEGF+CD24 | 2 | 10.19 | 0.94 | 100 | 80 | 93 |
| CK19 + CD24 | 2 | 18.56 | 0.61 | 40 | 100 | 60 |
| Signatureb | 2 | 7.19 | 0.96 | 100 | 80 | 93 |
| Signature+CK19 | 3 | 9.49 | 0.96 | 100 | 80 | 93 |
| Signature+CD24 | 3 | 10.52 | 0.96 | 100 | 80 | 93 |
| Signature+CK19 + CD24 | 4 | 38.86 | 0.91 | 80 | 100 | 80 |
Note: a Cut-off value was calculated using Youden’s index, which maximizes the sum of sensitivity and specificity; b Combined marker of VEGF and CD133; n number of biomarker(s); AUC area under the curve; NA not applicable