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. Author manuscript; available in PMC: 2020 Feb 5.
Published in final edited form as: Biochemistry. 2000 Jan 11;39(1):86–91. doi: 10.1021/bi991903b

Table 1:

Fluorescence Quenching and Anisotropy of AEDANS–TnI

labeled
position
complex C2+ Ksv
(M−1)
τ
(ns)
kq × 10−9
(M−1 s−1)
r(0) θ
(deg)
Cys-96 Tn–Tm yes 4.3 15.5 0.28 0.234 26.5
no 5.1 16.2 0.31 0.198 32.6
thin filament yes 4.3 15.1 0.28 0.234 26.5
no 3.7 13.4 0.28 0.267 23.4
Cys-117 Tn–Tm yes 3.7 17.2 0.21 0.225 29.2
no 4.2 17.4 0.24 0.176 35.1
thin filament yes 2.6 17.3 0.15 0.337 9.1
no 2.1 18.0 0.11 0.287 20.3