Fig. 2.

Transport of fluorescein isothiocyanate (FITC) encapsulated in Tf-coated nanoparticles across the artificial blood-brain barrier formed by BEND3 cells. The extent of transport was assessed by quantifying the fluorescence intensity of FITC using a fluorescent spectrophotometer at an excitation wavelength of 485 nm and an emission wavelength of 528 nm. All points represent the mean ± SEM, and any points without error bars indicate instances in which the SEM is encompassed by the data point. Abscissae: Hours following the introduction of the FITC dye to the left compartment of the Ussing chamber. Ordinates: Percentage of FITC that crossed to the right side of the Ussing chamber normalized to the amount remaining on the left side. All assessments were conducted in triplicate.