FIGURE 2:
INS-1 cells were transfected as in Figure 1 with the indicated cargoes. Fluorescence within the TGN region (A) and the ER (B) was monitored and the data were fitted using a first-order kinetic model to extrapolate ER (kER) and TGN rate constants (C). Representative curves are shown for each cargo (n = 16 for NPY-emdGFP-SBP from three independent transfections; n = 8 for SgII-emdGFP-SBP from two independent transfections; n = 14 for phogrin-emdGFP-SBP from three independent transfections; n = 11 for VMAT2-emdGFP-SBP from two independent transfections).