FIGURE 3:

Chlamydomonas metaphase spindles contain numerous mcMTs whose pole-proximal ends lie among the bundles of KMTs. (A) Model showing KMTs (yellow) and mcMTs (light blue). Pink circles mark the positions of mcMT pole-proximal ends that lie within 50 nm of a KMT (n = 62). (B) Model showing KMTs (yellow) and positions of close approach of a mcMT end (pink circles). Bar in A and B = 500 nm. (C) Histogram showing the frequency of places where mcMTs end ≤50 nm from a KMT as a function of distance from the spindle pole. (D) Examples of capped mcMTs (light blue) whose pole-proximal end lies near KMTs (yellow). Arrows indicate connections between these MTs. Bar = 150 nm. Below each panel is a cartoon showing our interpretation of the interMT connections.