Movie S1.

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Serial tomographic slices through a volume containing part of a Chlamydomonas metaphase spindle. The tomographic volume was built from four, 250 nm sections. The nuclear envelope, chromosomes, basal bodies and numerous spindle MTs can be seen. A 3D projection of the model shows KMTs (yellow) and mcMTs (light blue). Red indicates MTs that either end before reaching the chromosomes or end outside the reconstructed volume. The duplicated basal bodies are at the plasma membrane, not at the spindle pole. Scale bar = 500nm.