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. 2020 Jan 9;21(2):e48781. doi: 10.15252/embr.201948781

Figure 3. Smad4 expression is increased in mouse diabetic nephropathy (DN).

Figure 3

  • A–H
    Confocal microscopy demonstrated synaptopodin (A, Synap, green), Smad4 (B, red), DAPI (C, blue), merged image (D, Synap+Smad4+DAPI), digital enlargement of part of D (E), Tom20 (F, green), merged image (G, Smad4+Tom20+DAPI) and digital enlargement of part of G (H) in normal mouse kidney. Scale bars: 30 µm.
  • I–P
    Confocal microscopy demonstrated synaptopodin (I, Synap, green), Smad4 (J, red), DAPI (K, blue), merged image (L, Synap+Smad4+DAPI), digital enlargement of part of L (M), Tom20 (N, green), merged image (O, Smad4+Tom20+DAPI) and digital enlargement of part of O (P) in mouse DN (I–P). Scale bars: 30 µm. Of note, localization of Synap and Smad4, and Smad4 and Tom20.
  • Q
    Nephrin (+) cells were isolated by FACS from mouse kidneys.
  • R, S
    Western blotting demonstrated Smad4 expression in isolated nephrin (+) cells.
Data information: Unpaired t‐test was performed, n = 3. Data are shown as mean ± SD.