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. 2019 Dec 12;21(2):e48769. doi: 10.15252/embr.201948769

Figure 2. Mitochondrial Gasz interacts with Armi and Piwi bound with piRNA precursor.

Figure 2

  1. Armi and Piwi co‐immunoprecipitated with Flag‐Gasz (F‐Gasz) from the mitochondrial fraction (mito fraction) of OSCs (input). F‐Gasz was ectopically expressed in the cells by transfection prior to immunoprecipitation. *: Heavy chains of antibodies used for immunoprecipitation. n.i.: Non‐immune IgG used as a negative control.
  2. Co‐immunoprecipitation from whole‐cell lysate of OSCs before (control) and after (Zuc KD) Zuc depletion and subsequent Western blotting show that the levels of Piwi and Armi in the Flag‐Gasz (F‐Gasz) complex increased upon Zuc depletion (Zuc KD). Armi signal with a longer exposure is shown in Fig EV2A.
  3. Northern blotting shows that the flam‐piRNA precursors in the Flag‐Gasz complex (Flag) accumulated upon Zuc depletion (Zuc KD). A gel image of the control lanes (control) with a long exposure is shown in Fig EV2B.
  4. Upper: The flam‐piRNA precursors associated with Piwi disappeared upon Armi depletion (Armi KD) in Zuc‐lacking OSCs (Zuc KD). Lower: The abundance of immunopurified Piwi is shown by Western blotting.