Figure 3. Departure of Armi from Yb bodies depends on Piwi.

• A, B
  Depletion of endogenous Piwi (Piwi KD) in Zuc‐lacking OSCs (Zuc KD) caused Armi (green) to relocate to Yb bodies. Localization of Armi at Yb bodies is shown in (B). Mitochondrial (upper panel) and Yb (lower panel) signals are shown in red. The scale bar represents 5 μm. DAPI (blue) shows the nuclei.
• B
  The Armi complex was immunopurified from Zuc‐lacking OSCs (Zuc KD) before (control) and after (Piwi KD) Piwi depletion and probed for detecting Armi and Gasz. Armi no longer stably associated with endogenous Gasz upon Piwi depletion in Zuc‐lacking OSCs. Co‐IP index (*) shows that the ratio of Gasz amount in the Armi complex isolated from Zuc‐depleted cells (Zuc KD/control) and Zuc‐ and Piwi‐depleted cells (Zuc KD/Piwi KD) is 1:0.05.
• C
  The Flag‐Gasz (F‐Gasz) complex was immunopurified from Zuc‐lacking OSCs (Zuc KD) before (control) and after (Piwi KD) Piwi depletion and probed for detecting Armi and F‐Gasz. F‐Gasz was ectopically expressed prior to immunoprecipitation. Co‐IP index (*) shows that the ratio of Armi amount in the F‐Gasz complex isolated from Zuc‐depleted cells (Zuc KD/control) and Zuc‐ and Piwi‐depleted cells (Zuc KD/Piwi KD) is 1:0.31.
• D
  In vitro pull‐down assays show that Armi‐Flag (Armi‐F) directly binds with GST‐GaszΔC113 but hardly with GST. Armi‐F was immunopurified from Schneider 2 (S2) cells under harsh conditions. GST and GST‐GaszΔC113 were visualized by CBB staining, while Armi‐F was detected by Western blotting using anti‐Flag antibodies.