Figure EV3. Departure of Armi from Yb bodies depends on Piwi.

1. Larger immunostaining views of the cell images shown in Fig 3A and B. The cells appeared in the main figures are boxed with white dotted line. Armi behaved similarly in the cells other than the one shown in Fig 3A and B. The scale bar represents 5 μm.
2. Piwi depletion hardly affected the level of Armi in Zuc‐depleted OSCs. β‐Tubulin (β‐Tub) was detected as a loading control.
3. Myc‐Gasz (M‐Gasz) and Armi‐Flag (Armi‐F) interacted with each other in Schneider 2 (S2) cells.
4. Purified recombinant GST‐Gasz (ΔC113 lacking Leu349‐Ser461 containing the TM region at the C‐terminus) used in the experiment shown in Fig 3E. The protein was stained with CBB.