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. 2019 Dec 12;21(2):e48769. doi: 10.15252/embr.201948769

Figure EV4. Departure of Armi from Yb bodies requires piRNA precursor loading onto Piwi.

Figure EV4

  1. Upper: Another set of cell images of the one shown in Fig 4B. The scale bar represents 5 μm. DAPI (blue) shows the nuclei. Lower: The expression levels of Flag‐Piwi (F‐Piwi) WT and PAZ/MID mutants (PAZmt/MIDmt) in Zuc/Piwi‐depleted OSCs (Zuc KD + Piwi KD). β‐Tubulin (β‐Tub) was detected as a loading control.
  2. Upper: Armi localization to Yb bodies observed in Zuc/Piwi‐depleted OSCs (Zuc KD + Piwi KD) (Fig 3A and B) was rescued by ectopic expression of Flag‐Piwi WT and PAZmt but not of MIDmt. “Rescued” means that Armi is now localized to mitochondria as in Zuc‐depleted OSCs (Fig 1C). The scale bar represents 5 μm. DAPI (blue) shows the nuclei. Lower: Another set of cell images of the one shown on the left. The scale bar represents 5 μm. DAPI (blue) shows the nuclei.
  3. Upper: The cellular localization of Flag‐Piwi (F‐Piwi) WT and PAZ/MID mutants (PAZmt/MIDmt) (yellow), Yb (red), and Armi (green) in Piwi‐depleted OSCs (Piwi KD). Middle: Another set of cell images of the one shown on the left. The scale bar represents 5 μm. DAPI (blue) shows the nuclei. Lower: The expression levels of Flag‐Piwi (F‐Piwi) WT and PAZ/MID mutants (PAZmt/MIDmt) in Piwi‐depleted OSCs (Piwi KD). β‐Tubulin (β‐Tub) was detected as a loading control.
  4. Armi has three functions. [A] Armi's Yb‐body localization is required for Piwi localization at Yb bodies. [B] Armi senses and binds Piwi–pre‐piRISC at Yb bodies. This action of Armi is required for inter‐organelle translocation of the complex from Yb bodies to mitochondria. [C] Armi relaxes piRNA precursors for Zuc cleavage on the mitochondria.