Figure EV6. The N‐terminal end of Armi plays an important role in the departure of Piwi–pre‐piRISC from Yb bodies.

1. Anti‐Armi antibody 2F8A9 recognized both Armi bands on Western blots, while 2D6E11 recognized solely the upper band. The signals of 2F8A9 were stripped away, and then, the same membrane was reprobed with 2D6E11. β‐Tubulin (β‐Tub) was detected as a loading control.
2. Western blotting showed that 2F8A9 anti‐Armi antibody recognized both Armi‐Flag (Armi‐F) WT and ΔN34 mutant, while 2D6E11 recognized only WT. β‐Tubulin (β‐Tub) was detected as a loading control.
3. Left: Another set of cell images of the one shown in Fig 6B. The scale bar represents 5 μm. Right: The expression levels of Armi‐Flag (Armi‐F) WT and ΔN34 mutants in Zuc/Armi‐depleted OSCs (Zuc KD + Armi KD). β‐Tubulin (β‐Tub) was detected as a loading control.
4. Left: Armi‐Flag (Armi‐F) WT and the ΔN34 mutant were expressed in OSCs where endogenous Armi and Zuc had been depleted by RNAi (Zuc KD + Armi KD). Armi‐F WT (green) localized onto mitochondria, whereas Armi‐F ΔN34 mutant (green) localized to Yb bodies (red). The scale bar represents 5 μm. DAPI (blue) shows the nuclei. Right: Another set of cell images of the one shown on the left. The scale bar represents 5 μm. DAPI (blue) shows the nuclei.