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. 2019 Dec 12;21(2):e48597. doi: 10.15252/embr.201948597

Figure EV5. Methylation of LSD1 promotes migration and invasion of MDA‐MB‐231, MCF7, and MCF10A cells.

Figure EV5

  • A, B
    Wound‐healing assay of MM‐231‐shLSD1‐WT/R838A/R838K/R838F cells (A) and MCF7‐LSD1‐WT/R838A/R838K/R838F cells (B) with or without MS049 treatment. The wounds were created by scraping the cells with sterile pipette tip. Scale bars, 100 μm.
  • C, D
    Migration (C) and invasion assays (D) were conducted in MCF10A cells that co‐expressed CARM1 with LSD1 WT, or one of LSD1 mutants (R838A/R838K/R838F). Representative images of migrated and invaded cells are shown. Data represent the number of cells derived from mean cell counts of five fields (Error bars represent mean ± SD, n = 3 experimental replicates, *P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant, Student's t‐test).
  • E
    Wound‐healing assays of MCF10A cells that co‐expressed CARM1 with LSD1 WT, or one of LSD1 mutants (R838A/R838K/R838F). The wounds were created by scraping the cells with sterile pipette tip. Scale bars, 100 μm.

Source data are available online for this figure.