Gene	Sequence
TMEM16A
Forward primer	5′‐GTGGAGTCATACTGGAACATGTAG‐3′
Probe	5′‐/56‐FAM/AGAATCCAG/ZEN/AAACAAAGAGACCGACAAGG/3IABkFQ/‐3′
Reverse primer	5′‐GCCAGAGTCTTAGAGAAGTCAC‐3′
TMEM16B
Forward primer	5′‐GTGGAGTCATACTGGAACATGTAG‐3′
Probe	5′‐/56‐FAM/AGAAACATG/ZEN/GTAGCCCACAGAGCC/3IABkFQ/‐3′
Reverse primer	5′‐CCACCGTCTTCTTTTCCATCT‐3′
BEST1
Forward primer	5′‐GGCATCACTCATAGAGAACAGG‐3′
Probe	5′‐/56‐FAM/AGCATGCAG/ZEN/AGTCCTATCCCTACAGG/3IABkFQ/‐3′
Reverse primer	5′‐CCCCACAGAACATCTTCAAC‐3′
GAPDH
Forward primer	5′‐GTGGAGTCATACTGGAACATGTAG‐3′
Probe	5′‐/56‐FAM/TGCAAATGG/ZEN/CAGCCCTGGTG/3IABkFQ/‐3′
Reverse primer	5′‐AATGGTGAAGGTCGGTGTG‐3′