Figure 3. HO‐1^−/− mice have disturbed frequency of ECs, CARs, and MQs in BM and altered production of hematopoietic cytokines.

• A
  HO‐1^−/− mice have more ECs and less CARs (P = 0.053) in BM, while frequency of PαS does not differ.
• B
  Young HO‐1^−/− mice have lower frequency of Sca‐1^high fraction among BM ECs, what resembles phenotype of BM ECs in old mice.
• C, D
  Intracellular flow cytometry reveals (C) lower levels of SDF‐1α in HO‐1^−/− CARs and (D) lower levels of LAP protein in HO‐1^−/− ECs.
• E
  Analysis of cell surface SCF revealed lower frequency of SCF⁺ ECs in HO‐1^−/− while no differences among CARs.
• F, G
  The frequency of (F) CD163⁺ MQs and (G) CD163⁻ MQs in BM of HO‐1^−/− mice is altered.

Data information: Where individual data points not shown n = 4/group; unpaired two‐tailed t‐test. *P < 0.05, **P < 0.01, ***P < 0.001. Data shown are mean ± SEM from a single experiment.