Figure 5. LT‐HSCs from young HO‐1^−/− mice possess transcriptional profile resembling aged LT‐HSCs.

1. RNA‐seq revealed 1,067 DEGs between young HO‐1^−/− and HO‐1^+/+ LT‐HSCs.
2. RNA‐seq revealed 595 DEGs between old HO‐1^−/− and HO‐1^+/+ LT‐HSCs.
3. Selected GOBP terms enriched in GSEA in young HO‐1^−/− LT‐HSCs.
4. Selected GOBP terms enriched in GSEA in old HO‐1^−/− LT‐HSCs.
5. Significant part of DEGs (31% total) in young HO‐1^−/− LT‐HSCs overlaps with DEGs identified in LT‐HSCs during physiological aging.
6. PC1 in PCA on selected 1,148 genes separated young HO‐1^+/+ LT‐HSCs from other groups, clustering the young HO‐1^−/− LT‐HSCs together with old LT‐HSCs.
7. Hierarchical clustering on the 20% of most correlated 297 genes with PC1 showed two major clusters that include young HO‐1^+/+ LT‐HSCs in one and old LT‐HSCs with young HO‐1^−/− LT‐HSCs in second.
8. Selected GOBP terms enriched in GSEA based on 297 identified genes.

Data information: Color key on the heatmaps represents gene expression (as z‐score among row).