Figure 1. Akt and Fak Signals are Highly Activated in Pten^−/− Thymocytes.

(A) Fak expression in Lin⁻Sca1⁺c-Kit⁺ (LSK), LSKFlk2⁻, LSKFlk2⁺, CMP, GMP, MEP, CLP, Gr1⁺Mac1⁺, Ter119⁺, and CD41⁺ cells isolated from BM; F4/80⁺, B220⁺ cells from SP; and CD4⁻CD8⁻, CD4⁺CD8⁺, CD4⁺CD8⁻, CD4−CD8⁺ cells from TH of WT mice. (B) Fak and Akt activities in WT and Pten^−/− thymocytes. Controls: thymocytes from mice without pI:C injection; WT tail tip fibroblasts (TTF). Images representative of three independent experiments. Quantification (Multi Gauge 3.0) relative to WT control after normalized to loading controls. (C&D) pFak^Y397 in T-lymphocytes measured by FACS (C), and quantified by mean fluorescence intensity (MFI) (D). Data shown are Mean ± S.E.M. * compared to WT, P< 0.001, by Student’s t-test.