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. 2019 Aug 1;1(4):278–285. doi: 10.1016/j.jhepr.2019.07.006

Fig. 2.

Fig. 2

Disappearance rate and internalization of Myrcludex B. (A-B) Representative western blots (A) and quantification (B) of NTCP protein expression in a pulse-chase experiment using biotin in parental U2OS (P) and U2OS-HA-hNTCP cells. Western blots are representative of 5 independent experiments. Full uncropped blots are shown in Fig. S1. (C) Fluorescence intensity of Myrcludex B-FITC on U2OS-HA-hNTCP cells measured by Clariostar. (D) NTCP protein expression in U2OS-HA-hNTCP cells treated with vehicle or 200 nM Myrcludex B prior to the pulse chase experiment. Western blots are representative of 2 independent experiments. All data are represented as mean ± SD, *p ≪0.05 compared to the control determined by one-way ANOVA with Bonferroni post hoc analysis. FITC, fluorescein isothiocyanate; HA, hemagglutinin; NTCP, sodium taurocholate co-transporting polypeptide.