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. 2019 Dec 11;7(3):1901455. doi: 10.1002/advs.201901455

Figure 5.

Figure 5

PYLs interact with PP2ACs. a) A yeast two‐hybrid assay showing PYL1 interacting with PP2AC3/C4 and ABA enhancing this interaction. 2D synthetic dropout medium lacks Trp/Leu and 4D selective medium lacks Trp/Leu/His/Ade. 50 × 10−6 m ABA was applied. b) A co‐IP assay showing PYL1 interacting with PP2AC3/C4. As a negative control, PYL1 did not coimmunoprecipitate with RCN1. Treatment with 10 × 10−6 m ABA for 4 h led to enhanced interaction between PYL1 and PP2AC3/C4. Numbers under lanes indicate relative band intensities normalized to the loading controls. + and − indicate incubated with or without extracts or ABA treatment. c,d) Increased tolerance of pp2ac3 and pp2ac4 roots in response to ABA. The root gravitropic index (c) and lateral root density (d) were analyzed (n ≥ 10 roots). Data are means ± SE, (∗∗) P < 0.01 (Student's t‐test). Three independent experiments were performed with similar results. Representative images are shown.