FIGURE 1.

A limited screen for candidate interacting proteins. This schematic depicts the use of isolated stage 6 Xenopus oocytes to test whether a protein of interest (ephrinB1) is able to interact with possible candidate proteins (cell–cell adhesion and cell boundary proteins). Oocytes are injected with in vitro transcribed mRNA encoding ephrinB1 and individual candidate gene mRNAs, cultured for several hours, lysates prepared and ephrinB1 immune-complexes collected. Western analysis should then be performed to detect whether a candidate associates with the ephrinB1 protein