KEY RESOURCES TABLE
| Reagent or Resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Mouse Anti-human IgG1 Fc-HRP | Southern Biotech | Cat#9054–05; RRID: AB_2687484 |
| 4A5, Alexa 647 conjugated | In house | N/A |
| IgG2b, A647 conjugated isotype control mAb | BD Biosciences | Cat#557903; RRID: AB_396928 |
| Rabbit Anti-HS1 (human specific) | Cell Signaling Technology | Cat#4503S; RRID: AB_2096977 |
| Rabbit polyclonal Anti-phospho HS1 (Y378) | OriGene | Cat#TA314001 |
| Mouse monoclonal anti-RhoA | Cytoskeleton | Cat#ARH04 |
| Anti-mouse IgG, HRP-linked | Cell Signaling Technology | Cat#7076; RRID: AB_330924 |
| Anti-rabbit IgG, HRP-linked | Cell Signaling Technology | Cat#7074; RRID: AB_2099233 |
| Human IgG Control Ab | Sigma | Cat#I5154; RRID: AB_1163610 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ROR1-extracellular domain | In house | N/A |
| Rhotekin-RBD beads | Cytoskeleton | Cat#RT02 |
| Quantum MESF Kit, Alexa Fluor 647 | Bangs Laboratories | Cat#555p |
| Deposited Data | ||
| Raw and analyzed RNA-seq data | NCBI GEO | GEO: GSE114382 |
| Software and Algorithms | ||
| Gel-Pro Analyzer 4.0 software | Media Cybernetics | http://www.mediacy.com |
| FlowJo (v.2.7.4) | FlowJo | https://www.flowjo.com |
| Gene set enrichment analysis | Broad Institute | http://software.broadinstitute.org/gsea/index.jsp |
| QuickCal v.2.3 analysis template | Bangs Laboratories | https://www.bangslabs.com/ |
| Graphpad Prism v.6.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| TMB microwell peroxidase substrate kit | SeraCare | Cat#5120–0053 |
| BSA | Fisher | Cat#BP1600 |
| ELISA high-protein binding plates | Costar | Cat#3690 |
| WNT5A ELISA kit | Aviva Systems Biology | Cat#OKEH00723, RRID: SCR_013560 |
| Cirmtuzumab | Pacific GMP/Abzena | N/A |