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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Mol Neurosci. 2019 Sep 13;70(1):102–111. doi: 10.1007/s12031-019-01405-w

Fig. 6. Confirmation of knockout.

Fig. 6

(A-C) Film autoradiograms showing hemisections at the level of the ventral midbrain hybridized with either antisense Slc6a8 cRNA, (D-F) TH cRNA or (G-I) control sense Slc6a8 cRNA. Robust Slc6a8 cRNA-labeling is present throughout the sections from the (A) Slc6a8+/y and (B) dCrt−/y mice; note, however, the absence of hybridization signal in the substantia nigra in the dCrt−/y brain section (arrows in B; compare to same area in A). (C) Labeling for Slc6a8 mRNA is absent (only typical background expression) in sections from Slc6a8−/y mouse brains. Panels D-F show TH cRNA labeling in sections semi-adjacent to panels A-C, respectively, and serve to identify the corresponding localization of dopaminergic cells in the ventral midbrain. Hybridization of semi-adjacent sections from each mouse genotype with the control sense Slc6a8 cRNA probe reveals complete absence of labeling for Slc6a8 mRNA (G-I)