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Timeline of experimental design. For 2 weeks prior to prolonged jaw opening, some animals were fed either 0.5% ECBB or 0.1% homemade broth. Baseline nociception measurements were obtained (prior to feeding), and additional assessments were collected at 2 hours and 3 and 7 days after the prolonged jaw opening. Animals receiving either broth were allowed to consume the product for 1 week after jaw opening (for a total of 3 weeks of broth consumption).
