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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2019 Oct 8;43(11):2332–2343. doi: 10.1111/acer.14200

Figure 8: In dams and fetuses, maternal dietary iron fortification mitigated the elevated STAT3 in response to PAE.

Figure 8:

PAE elevated p-STAT3 in maternal (A) and fetal (B) livers, and IF normalized p-STAT3 levels to IS-M control levels, even in the presence of PAE. Values for IS-M and IS-PAE are reproduced from Figures 1D (maternal) and 3D (fetal), as these data were generated simultaneously. Bar graphs are means ± SEMs. For maternal data, N = 7–9 individual animals for IS measurements and N= 4 individual animals for IF measurements. For fetal data, N=2 fetuses per litter/6 litters for IS measurements and N=2 fetuses per litter/3 litters for IF measurements. The protein quantification of maternal and fetal IF-M and IF-PAE p-STAT3/STAT3 is represented relative to IS-M p-STAT3/STAT3 abundance from Figures 1 and 3 after normalization to GAPDH abundance within the same lane. Statistics were conducted as in Figure 1. Means are significantly different when they do not share a common letter, P<0.05. Data lacked outliers as determined by Grubb’s test. IS, iron-sufficient; IF, iron-fortified; M, maltodextrin; PAE, prenatal alcohol exposure.