Fig 3. Mkrn1 mutations affect accumulation of proteins involved in axis patterning.

(A-D) Posterior accumulation of Osk is greatly reduced in stage 10 Mkrn1^W and Mkrn1^S oocytes as compared with wild-type. Osk is nearly undetectable in Mkrn1^N egg chambers. Scale bars, 25 μm. (E-H) Posterior accumulation of Stau is greatly reduced in stage 10 Mkrn1^W and Mkrn1^S oocytes as compared with wild-type. Stau is nearly undetectable in Mkrn1^N egg chambers. Scale bars, 25 μm. (I-L) Anterodorsal accumulation of Grk is normal in stage 10 Mkrn1^W oocytes. Grk remains associated with the oocyte nucleus and is mislocalized to the posterior in stage 10 Mkrn1^S oocytes. Grk is present at uniformly low levels or undetectable levels in all germ cells in Mkrn1^N egg chambers. Scale bars, (I-K) 20 μm, (L) 25 μm. (M-P) Posterior accumulation of Aub is greatly reduced in stage 10 Mkrn1^W and Mkrn1^S oocytes as compared with wild-type. Aub is present at uniform levels in all germ cells in Mkrn1^N egg chambers. Scale bars, 20 μm. (Q-T) Posterior accumulation of Vas is greatly reduced in stage 10 Mkrn1^W and Mkrn1^S oocytes as compared with wild-type. Vas is present at uniform levels in all germ cells in Mkrn1^N egg chambers. Scale bars, 25 μm. (U-X) Accumulation of Orb is similar in wild-type and Mkrn1^W oocytes, but Orb is more concentrated in the posterior of Mkrn1^S oocytes. In early-stage Mkrn1^N egg chambers there is usually a single Orb-positive cell, indicating that some steps toward oocyte differentiation are able to take place. Scale bars, (U-W) 20 μm, (X) 25 μm.