Fig. 2.

HA accumulates in islets from a subset of aAb⁺ donors. HA staining (brown) in islets from (a) aAb⁻, (b, c) single-aAb⁺ and (d) double-aAb⁺ donors. Arrowheads point to the islet border. Scale bars, 50 μm. (e) Morphometric quantification of islet HA⁺ areas, shown on a log₁₀ scale. Each circle denotes an individual donor; blue, aAb⁻; light red, single-aAb⁺; dark red, double-aAb⁺. Data are mean values of islet HA⁺ areas for each individual donor. The numbers (1–7) indicate the aAb⁺HA^high tissues ranked according to the size of islet HA⁺ areas. The dotted horizontal line indicates the upper cut-off value (mean of HA⁺ area in aAb⁻control + 3 SD). In total, 4598, 3210 and 2272 islets from aAb⁻, single-aAb⁺ and double-aAb⁺ donor tissues were analysed, respectively. (f) Islet HA⁺ area size distribution. The pie charts represent the percentage of islets with HA⁺ areas falling within each of the HA⁺ area size categories. Islets were analysed in aAb⁻ tissues (n=4598 islets) and in aAb⁺HA^high tissues from n=3 single-aAb⁺ donors (n=982 islets) and n=4 double-aAb⁺ donors (n=1828 islets). *p<0.001,single-aAb⁺HA^high vs controls, and double-aAb⁺HA^high vs single-aAb⁺HA^high or controls; Mann–Whitney U test